While comparing Sphene and Biotite physical properties, the important data you should know is its chemical composition. Since chemical formula defines the molecular structure of the crystal, most of the physical properties like color, tenacity, solubility are governed by Sphene and Biotite chemical formula.
Another important criteria for qualitative analysis of gemstones is Sphene and Biotite Specific gravity. Specific gravity is the relative density of a gemstone compared with respect to density of water. Gemologists use Sphene and Biotite Optical Properties during the identification of gemstone. Specific gravity of Sphene is 2.7-3.3 whereas that of Biotite is 2.7-3.3.